肺癌EB病毒LMP1基因片段的检测及变异株的意义初探

Detection of EB virus LMP1 fragment and the preliminary exploration of the significance of its mutation in pulmonary carcinoma

目的:研究ＥＢ病毒ＬＭＰ１基因与肺癌的关系,并对其变异株进行分析。方法:应用多聚酶链反应检测了４０例肺癌ＥＢＶ－ＬＭＰ１基因片段,并对其进行克隆及测序分析。结果:４０例肺癌标本中,１１例(２７．５%)阳性;对１１例ＥＢＶ－ＬＭＰ１ ＤＮＡ阳性产物用限制性内切酶ＳｔｙⅠ酶切消化,发现９例与标准病毒完全一致,２例不同,提示ＥＢＶ－ＬＭＰ１基因有变异存在。结论:ＥＢ病毒的感染及ＬＭＰ１基因片段的变异在肺癌的发生、发展中可能发挥着一定的作用。

ve To study the relationship between EB virus LMP1 fragment and pulmonary carcinoma and assess the significance of the mutation. Methods EBV- LMPl fragments were amplified in 40 cases of pulmonary carcinoma by PCR and then cloned and sequenced. Results In 40 cases of pulmonary carcinoma, 11 cases (27. 5%) were positive. These 11 cases of positive products were then analyzed with restrictive enzyme sty I . Among them, 9 cases were the same with standard EBV DNA.and the other 2 cases existed mutation. Conclusion The EBV infection and the mutation of LMP1 fragment may play an important role in the genesis and development of pulmonary carcinoma.