正常脾脏与肝硬化脾脏组织基因表达概况分析

Gene expression profile in normal and cirrhotic spleen tissues

目的 了解正常脾脏与肝硬化脾脏的基因表达概况 ,寻找在两者之间的差异表达基因。方法 以正常脾脏及肝硬化脾脏组织的总RNA反转录合成含有α 3 2 PdATP的cDNA为探针 ,与Atlas微阵列表达分析膜进行差异杂交。结果 放射自显影结果经AtlasImageTM 软件分析显示 :在所分析的 5 88种已知基因中 ,CK8、ICE LAP6、PISSLARE等 32个在肝硬化脾脏组织中表达上调 ,byglycan、Caspase 10、CRAF 1等 45个表达下调 ,参与细胞增殖、凋亡、分化、细胞间相互作用 ,与细胞侵袭相关的基因表达水平发生了明显改变。结论 通过Atlas微阵列系统分析发现的这些基因的表达改变组成了一个不同情况下脾脏特异的基因表达谱 ,是人类首次全面系统地研究脾脏的基因表达改变 ,一些与脾脏变化有关的差异表达基因 ,对彻底了解脾脏在肝硬化过程中的变化及其所发挥的作用有重要的意义。Atlas微阵列技术的应用将为人类了解疾病的发生、发展过程提供一个较好的方法。

Objective To describe a spleen specific gene expression profile and identify genes showing expression differences between normal and cirrhotic spleen tissues. Methods The cDNA probe labeled with α 32 P dATP was synthesized from total RNA of 28 samples of normal and cirrhotic spleen tissues. Then it was separately hybridized to two identified Atlas human cancer cDNA expression array membranes containing 588 known genes. Results Autoradiographic results were analyzed by specific AtlasImage TM (version 1 01a) software. Amongst the 588 genes, 32 including CK8, ICE LAP6 and PISSLARE etc. were up regulated and 45 including byglycan, Caspase 10 and CRAF 1 etc. were down regulated in cirrhotic spleen. The most significant changes were found in expression levels of genes associated with the regulation of cell proliferation, apoptosis, differentiation and cell cell interaction as well as invasion regulators and cytokines. Conclusions The results obtained from Atlas microarray provide a comprehensive spleen specific gene expression profile in different situations. Some modified genes may be helpful for finding the effect of spleen on liver cirrhosis. Microarray is a useful method to find the onset and progression of many diseases.