Tapasin对HLA-E细胞表面表达的影响

The influence of tapasin on HLA-E expression on cell surface

目的 :探索伴侣分子Tapasin对HLA E分子细胞表面表达的影响。方法 :体外构建HLA EcDNA的逆转录病毒表达载体 ,并通过感染的方法将其转入Tapasin阴性的T2细胞系 ,利用流式细胞术检测HLA E分子在靶细胞表面的表达情况。结果 :外源HLA E基因在Tapasin阴性的T2细胞表面获得表达 (74 13% ) ,而对照细胞及经空载体转染的T2细胞表面未检测到HLA E分子的表达。结论 :HLA E分子的细胞表面表达也存在TAP非依赖的方式。

Objective:To explore the influence of chaperone tapasin on HLA E expression on cell surface.Methods:The infection method was used to transfer the recombinant retrovirus expression vector which was constructed with HLA E molecules on target cells was detected with FACS technique.Results:Exogenous HLA E expression on T2 cell surface was detected with 74.13% and no HLA E expression was detected on control cells and that was transfected with empty vector.Conclusion:It is possible that the expression of HLA E molecules on cell surface involves in TAP independent mechanism.