MTS比色法检测嗜中性粒细胞产生的活性氧

A colorimetric Assay for Determination of Reactive Oxygen Produced by Activated Neutrophils with MTS

目的 建立一种简便可靠的吞噬细胞“呼吸爆发”检测方法。方法 以 MTS为受氢体检测嗜中性粒细胞受激发后产生的超氧离子 ,从而间接反映其“呼吸爆发”发生情况。结果 该法与常用的高铁细胞色素 C还原法有良好的相关性 ,Y( MTS法 ) =2 .3 0 7X- 0 .2 91 ,r=0 .9941。 1 9例反复感染患者的嗜中性粒细胞的活性氧产量明显低于健康人。结论 该法是一种简便可靠的吞噬细胞“呼吸爆发”检测方法 ,可用于吞噬细胞功能的检测。

Objective To establish a simple,valid assay for measurement of respiratory burst in phagocytes.Methods The superoxide anions produced by those neutrophils pretreated with stimulators were detected through MTS as hydrogen receptor,then the respiratory burst in the cells was calculated indirectly.Results MTS colorimetric assay was correlative to ferric cytochrome C reduction assay significantly (Y _ (MTS) =2.307 X -0.291, r =0.994 1).The reactivc oxygen in 19 patients with recurrent infection was lower than normal ones significantly.Conclusions This assay is a simple,valid assay for measurement of respiratory burst in phagocytes,can be used in determination for phagocyte function.