摘要
目的 探讨对抗 - HCV ELISA阳性血液进行确认分析的重要性。方法 用第三代免疫印迹法(RIBA3 .0 )对抗 HCV ELISA阳性血液进行确认 ,并对部分样品用荧光定量聚合酶链反应 (FQ- PCR)检测 HCV- RNA。结果 80份抗 HCV ELISA阳性血样经 RIBA确认 ,其中阳性 3 8份 (47.5 % ) ,可疑 2 0份(2 5 % ) ,阴性 2 2份 (2 7.5 % ) ,c2 2、c3 3 c的检出率高于 c- 1 0 0和 NS5,而且 2 0份 RIBA3 .0可疑血样的HCV- RNA为阴性。结论 对抗 - HCV阳性血液确认尤为重要 ,能提高诊断 HCV的准确性。
Objective To evaluate the importance of confirmatory test for detecting antibody against HCV ELISA(+).Methods Anti-HCV ELISA(+) samples were confirmed by RIBA 3.0,and some of them were detected by FQ-PCR.Results 80 anti-HCV ELISA(+) samples were confirmed by RIBA 3.0,38(47.5%)were positive,20(25%) were indeterminate but HCV-RNA of them were negative,and 22(27.5%) were negative.Also the positive rate of c22 and c33c was higher than that of c-100 and NS5.Conclusion The results show that anti-HCV ELISA(+) samples should be confirmed by RIBA 3.0 in order to ensure the accuracy of diagnose.
出处
《陕西医学检验》
2002年第1期31-32,共2页
Shaanxi Journal of Medical Laboratory Sciences