莫索尼定点眼对家兔葡萄膜巩膜途径房水流量的影响

Effect of topically applied moxonidine on uveoscleral outflow∥Zhang Lei, Yang Jiangang, Sun Naixue

目的 测定莫索尼定 (Moxonidine ,MOX)局部应用后对家兔葡萄膜巩膜途径 (Uveoscle raloutflow ,Fu)房水流出量的影响 ,探讨MOX局部应用的房水动力学机制。方法 正常家兔2 0只 ,随机分为 2组 ,分别采用生理盐水及莫索尼定点眼 ,点眼前及点眼后 3 0min ,测量双眼眼压。点眼后 1 .5h用 0 .1mmol/L异硫氰酸荧光素牛血清白蛋白 (Fluorescein isothiocyanatebovinealbumi,FITC BSA)行前房灌注 ,测定Fu各组织的荧光强度 ,采用改良Suguro法计算各组Fu房水再现量。结果 MOX单侧点眼后 ,点眼侧兔眼Fu房水再现量为 (7.2 1± 0 .988)×1 0 - 1 μL·3 0min- 1 ,对侧眼Fu房水再现量为 (7.5 3± 0 .2 1 4)× 1 0 - 1 μL·3 0min- 1 ,与对照组相比具有显著性差异。

Objective To quantitatively analyze the effect of topically applied moxonidine on uveoscleral outflow.Methods Healthy white rabbits were randomly divided into 2 groups, 0.9% saline and moxonidine were applied unilaterally. Thirty minutes before and after the treatment, the intraocular pressure of bilateral eys was measured. One and half hours after the treatment, the eyes were perfused for 30 minutes with 0.1?mmol·L -1 FITC BSA. Then the eyes were enucleated and dissected into anterior and posterior uvea, anterior and posterior sclera, retina and remaining fluid. The uveosleral outflow was calculated by using modified Suguro method.Results Topically applied moxonidine could prominently increase the quantity of uveoscleral outflow in rabbits bilaterally. There was significant difference between the MOX group and the control group ( P <0.05). Furthermore, the contralateral eye and ipsilateral eye had no significant difference in their uveoscleral outflow( P >0.05).Conclusion Moxonidine's IOP lowering effect at least partly is through increasing uveoscleral outflow.