结合脂蛋白肽136-150修饰抗原肽与实验性变态反应脑脊髓炎

Altered peptide ligand of PLP136-150 and experiment allergenic encephalomyelitis

目的 观察结合脂蛋白肽 136 15 0 (PLP136 15 0 )修饰抗原肽是否具有引起脑脊髓炎(EAE)的可能性。方法 用 2 0 0 μgPLP136 15 0修饰抗原肽分组免疫SJL/J雌性小鼠 ,诱导主动EAE。对未发生主动EAE组小鼠再用已免疫抗原 5 0 μg重复接种 1次 ,继而建立短期T细胞株。用PLP136 15 0或修饰抗原肽 2 0 μg/ml分别刺激T细胞并将得到的T淋巴母细胞转移给同种小鼠 [(1～ 2 )× 10 7/鼠 ]以诱发被动EAE。结果 在 2 2个一位氨基酸被替换的修饰抗原肽中除 144A、K外 ,2 0个均成功诱发主动EAE ,一位氨基酸变异的 144A、K ,及双位 (143A/ 145A ,145A/ 148K)和三位 (139A/ 144A/ 148A)氨基酸被替换的修饰抗原虽不能引起主动EAE ,但若将这些抗原诱导、建立并刺激的T细胞株转移给同种小鼠可引起被动EAE ,而接受PLP136 15 0刺激的同种T细胞的小鼠不发生被动EAE。

Objective To find the potentiality of PLP136-150 altered peptide ligand(APL) of inducing EAE. Methods SJL/J female mice were immunized with 200μg of substituted peptides of PLP136-150. If there was no EAE occured, the mice would be rechallenged with 50μg of the same APL. Short-term T-cell lines were obtained from those animals.(1-2)×10 7 blast T-cells stimulated by PLP136-150 or its APL were transferred to homologous mice for inducing passive EAE. Results 20 of 22 APL which were substituted by one single amino acid but not 144A and K induced active EAE. T-cell lines premed and stimulated separately by 144A, K, double(143A/145A, 145A/148K) and three APL(139A/144A/148A) with three aa substitution induced passive EAE. Mice received the same kind of T cells stimulated by PLP136-150 did not developed EAE. Conclusion APL of PLP136-150 are potentially capable of inducing EAE.