卵清白蛋白特异性T细胞免疫诱导BALB/c小鼠的调节性免疫应答

T cell vaccination induces regulatory T cell response in BALB/c mice

目的 研究T细胞免疫后正常小鼠的调节性免疫应答。方法 应用体外扩增的卵清白蛋白 (OVA)特异的T细胞克隆免疫BALB c小鼠 ,3 H TdR掺入法分析细胞增殖 ,3 H TdR标记靶细胞检测杀伤T细胞的杀伤效应 ,间接免疫荧光法分析血清中抗T细胞抗体水平。结果 T细胞免疫后能诱导BALB c小鼠产生调节性T细胞的增殖反应、对靶细胞的杀伤效应以及针对于活化的T细胞的体液免疫应答 ,并进一步降低机体对OVA抗原的应答。

Objective To investigate regulatory T cell response induced by T cell vaccination. Methods BALB/c mice were vaccinated with activated OVA-specific T cell clones expanded in vitro. Cell proliferation was measured by 3H-TdR incorporation. CTL response was assayed by JAM test. Indirect immuno-fluorescent assay was used to examine the anti-T cell antibody level. Results T cell vaccination induced regulatory T cell response including T cell proliferation, CTL activity and antibody response targeting activated T cells. Conclusion Regulatory T cell response can be induced by T cell vaccination in normal mice.