肾综合征出血热患者外周血淋巴细胞Fas/FasL表达及与血清sFas/sFasL的相关性研究

Expression of Fas/FasL on peripheral blood lymphocytes and its relationship with serum sFas/sFasL in patients with hemorrhagic fever with renal syndrome

目的 探讨Fas FasL系统介导的细胞凋亡在肾综合征出血热 (HFRS)致病机理中的作用。方法 采用SABC免疫组化法检测HFRS病人外周血淋巴细胞Fas FasL的表达 ;采用ELISA法测定HFRS病人血清sFas sFasL的浓度。结果 HFRS病人发热期、低血压休克期、少尿期及多尿期外周血淋巴细胞Fas的表达明显增强 ,与正常对照组比较差异有极显著性 (P <0 .0 0 1) ;发热期、低血压休克期及少尿期淋巴细胞FasL的表达明显增强 ,与正常对照组比较差异有极显著性 (P <0 .0 0 1) ;HFRS病人各期血清sFas的浓度增高 ,与正常对照组比较差异有显著性 (P <0 .0 1) ,其中少尿期sFas的浓度最高 ,与其它各期比较差异有显著性 (P <0 .0 1) ;发热期与低血压休克期血清sFasL的浓度增高 ,与其它各期及正常对照组比较差异有显著性 (P <0 .0 1) ;淋巴细胞Fas及FasL的表达经直线相关分析(r=0 .885 5 ,P <0 .0 0 1) ,呈高度正相关 ;淋巴细胞FasL的表达与血清sFasL浓度经直线相关分析(r=0 .480 3,P <0 .0 1) ,呈高度正相关。结论 HFRS的致病过程中存在Fas FasL介导的细胞凋亡 ,sFas浓度的高低与机体损害的程度有关 ;淋巴细胞FasL的高表达及血清sFasL的浓度增高 。

Objective To study the Fas/FasL system-mediated cell apoptosis in the pathogenesis of hemorrhagic fever with renal syndrome(HFRS). Method SABC immunohistochemistry method was used to detect the expression of Fas/FasL on peripheral blood lymphocytes. Serum sFas/sFasL concentration was measured by ELISA. Results Expression of Fas on lymphocytes in the stages of fever, shock, oliguria and polyuria was significantly higher than that in normal controls (P<0.001); Expression of FasL on lymphocytes in fever, shock and oliguria stages was also significantly higher than that in normal controls (P<0.001). Serum sFas concentration in each stage of HFRS was higher than that in normal controls (P<0.01), and among them, serum sFas concentration in oliguria stage was the highest (P<0.01); Serum sFasL concentration in fever and shock stages was significantly higher than those in other stages and normal controls (P<0.01). Fas expression on lymphocytes has a positive relationship with FasL (r=0.885 5, P<0.001), and FasL expression on lymphocytes has a positive relationship with serum sFasL concentration (r=0.480 3, P<0.01). Conclusion Fas/FasL mediated cell apoptosis is an important factor in the pathogenesis of HFRS; sFas concentration is positively related to the tissue damage; High expression of FasL on lymphocytes and increased serum sFasL concentration may result in cell apoptosis, and thereby to clean out virus from host.