摘要
采用细胞生物学方法和组织培养技术研究小鼠胚胎原始生殖细胞(PGCs)的一般形态学特征及生物学特点.在体外培养条件下,PGCs 按体积大小可分大、中、小3类。本研究发现,中、小型原始生殖细胞在培养的第2、3 d 增多,形态似大型原始生殖细胞。培养中的 PGCs 不贴壁而悬浮生长于培养液中,培养到第6～7 d时开始死亡。体细胞(间充质细胞或间皮细胞)贴壁生长,生长旺盛,与PGCs 同时死亡。
The primordial germ cells (PGCs) of mouse embryo could be divided into three kinds by their size.It's defined that middle and small PGCs start increasing in two or three days. In vitro culture it looks like big PGCs.PGCs grow suspended in the 6th or 7th days.It's unknown that PGCs in vitro can propagate (reproduce).Somatic cells can grow luxumantly on the wall.Generally it dies at the same time as PGCs.
关键词
原始生殖细胞
体外培养
胚胎
小鼠
primordial germ cells
in vitro culture
mouse embryo
