斑点-ELISA与平板-ELISA诊断脑囊虫病的敏感性比较观察

A Comparative Study of Diagnosis for Cerebrocysticercosis by Dot-ELISA and Standard ELISA

应用斑点-ELISA 和平板-ELISA 对52例脑囊虫病患者的血清进行抗体检测。结果显示,两种免疫方法诊断脑囊虫病的敏感性近似(阳性率分别为92.3%与90.4%);与40例正常人血清均无假阳性反应。但斑点-ELISA 具有节省抗原,操作简便,不需特殊仪器设备等优点,适用于临床诊断及流行病学调查。

The dot enzyme-linked immunosorbent assay(Dot-ELISA)and standard enzyme- linked immunosorbent assay(ELISA)were used to detect Cysticercus cellulosae antibodies in the sera of the patients with cerebroeysticercosis.By using Dot-ELISA,47 out of 52 pa- tients(92.3%)showed a positive reaction.In the standard ELISA,47 out of 52 patients (90.4%)showed a positive reaction.None of the 40 serum samples from normal controls was found positive by both assays.Although there were similar higher sensitivity and speci- ficity in the diagnosis of cerebrocysticereosis by both Dot-ELISA and standard ELISA,the Dot-ELISA is more economical,simple and do not need any specific equipment for the diag- nosis of cerebrocysticercosis.Therefore,Dot-ELISA is suitable for clinic application and epi- demic survey.