摘要
应用 SDS-PAGE 分析了泡球蚴囊液及全囊、原头蚴和囊壁组织可溶性蛋白质,结果分别出现23、26、16、25条蛋白带,其中主带分别为4、7、5、7条。用斑点 ELISA 显示,4种抗原的敏感性分别为67%、67%、100%、67%,特异性分别为90%、89%、83.5%、91%。
The soluble proteins of cyst fluid,hydatid cysts,protoscoleces and cyst walls of alveolar hydatid obtained from experimentally infected gerbils(Meriones unguiculatus)were studied with SDS-PAGE.Four specimens showed respectively 23,26,26,and 25 protein bands with their major bands being respectively 4,7,5,and 7.Four kinds of soluble proteins men- tioned above were tested as antigens with Dot-ELISA.A sensitivity of 67%,67%,100%, and 67% were found while the specificity showed 90%,89%,83.5%,and 91% respective- ly.
