豚鼠过敏性休克IgE和C5复合物分布及c-kit蛋白表达

The Distribution of IgE and C5 Complement,the Expression of c-kit Protein in Anaphylactic Shock Guinea-pigs

【目的】探索过敏性休克法医学客观诊断标准。【方法】建立豚鼠异种血清过敏性休克模型 ,采用LSAB免疫组化方法检测模型鼠脏器IgE ,c kit蛋白以及C5复合体的分布。【结果】过敏性休克豚鼠肝 ,脾未检出IgE ,肺、肾检见大量IgE ,胃黏膜层c kit蛋白阳性细胞明显增多 ,各脏器均未检测出C5复合物。【结论】过敏性休克时 ,肺小血管壁及管周 ,肾小管内检见大量IgE ,可作为法医检案客观诊断标准。同时提示 ,肺和肾是病变最严重的脏器 ,补体C5未参与异种血清造成的过敏性休克过程。胃黏膜层c kit蛋白阳性细胞增多可作为辅助诊断指标。

Objective To investigate the distribution of IgE, c-kit protein and C5 complement in the guinea-pigs which suffered from human serum anaphylactic shock. Methods LSAB immuno-histochemical method was used.Results IgE was detected in lungs and kidneys but not in livers and spleens in anaphylactic guinea-pigs, The expression of c-kit increased in Anaphylactic guinea-pigs when compared with the control group. There was no C5 complement expression in both model and control groups. Conclusion The distribution of IgE in lungs and kidneys may be useful in establishing a diagnosis of anaphylactic shock.