摘要
将貂肠炎病毒中间复制型DNA(MEV—RF—DNA)的Hind Ⅲ酶切C片段克隆到pBR332中,形成重组质粒pBM.以光生物素标记的pBM探针,检测接种细小病毒前的貂和犬粪样36份和12份,阳性率分别为36.1%和33.3%;检测接毒后不同时间的貂和犬粪样98份和71份,阳性率分别是90.8%和91.5%.探针对接毒前、后貂和大粪样检出的阳性率均明显高于常规HA试验.另外,对部分貂和大粪样进行了电镜检查和病毒分离试验,并与探针检测结果进行了比较.
Hind Ⅲ C fragment of replicative form (RF) DNA of mink enteritis virus (MEV) was cloned into plasmid vector pBR322, and which designated as pBM. By dot hybridization, 36 mink and 12 dog fecal specimens before virus inoculation were detected with positive rate of 36. 1% and 33. 3%, 98 fecal specimens from MEV infected minks and 71 fecal specimens from canine parvovirus infected dogs were examined with positive rate of 90.8% and 91. 5% respectively. The results showed that the positive rate of the specimens detected with pBM probe was apparently higher than that of the standard hemagglutination test. For comparison with pBM probe detection, a part of the mink and dog fecal specimens were examined with electron microscopy and virus isolation.
关键词
貂
犬
细小病毒
感染
DNA
探针
mink
dog j parvovirus
photobiotin
DNA probe
dot hybridization