老年大鼠含淫羊藿血清对成骨细胞的增殖与分化的影响

Effects of serum of aged male rat treated with epimedium herb extract on proliferation and differentation of rat calvarium osteoblast in vitro

目的 观察老年雄性大鼠含淫羊藿血清对新生大鼠颅骨成骨细胞增殖和分化的影响。方法 雄性 ,19月龄SD大鼠 2 4只 ,随机分为 3组 ,分别为生理盐水组、淫羊藿低剂量组 (1g ml)、淫羊藿高剂量组 (2g ml) ,按 0 5ml 10 0g的量 ,每日两次 ,连续灌胃 1个月 ,处死前 1h ,再灌胃一次 ,取血清 (灭活 ,过滤 ) ,最终稀释成 1∶2 0、1∶4 0、1∶80加入新生大鼠颅骨成骨细胞体系 ,用MTT法检测细胞增殖 ,另外 ,分别在第 2天、第 3天、第 4天动态观察细胞生长过程 ,做生长曲线和检测分化指标ALP。结果 老年大鼠含药血清在 1∶80稀释比 ,MTT测得的OD值给药组高于对照组 (P <0 0 5 )且以高剂量组高于低剂量组 (P <0 0 5 ) ,生长曲线表现为 ,在第 2天各组无明显差别 ,从第 3天开始 ,含药血清增殖速度加快 ,仍以高剂量组最快。ALP检测结果 ,含药血清OD值高于对照组 (P <0 0 5 ) ,但不同剂量之间没有差异 (P >0 0 5 )。结论 老年雄性SD大鼠连续服用淫羊藿水提液 1个月 。

Objective\ To investigate the effects of serum of aged rat treated with extract of epimedium herb on proliferation and differentiation of rat calvarium osteoblast in vitro. Methods\ Twenty\|four SD rats at age of 19 months were randomly divided into 3 groups and treated with either saline or extract of epimedium herb (1 g/ml) and extract of epimedium herb (2 g/ml) respectively for 3 months. The animal serum was prepared for culture system in different dilution ratio (1∶20,1∶40,1∶80).MTT, alkaline phosphatase (ALP) activity were detected to determine the status and activities of proliferation and differentiation. Results\ The serum from the treated rats at dilution ratio of 1∶80 significantly induced cell proliferation and differentiation in a dose\|and time\|dcpendert manner, compared with the control( P <0\^05). Conclusion\ Serum of the aged rat treated with epimedium herb extract stimulates the proliferation and differentiation of neonatal rat osteoblast in vitro.