人脑胶质瘤组织中SV40 DNA及大T抗原研究

Research on simian virus 40 DNA and large T antigen in human brain glioma tissues

目的 观察猴病毒40（SV40）DNA及大T抗原（Tag）在人脑胶质瘤的定位表达情况,探讨其在胶质瘤发生发展中的生物学意义。方法 采用原位杂交和免疫组化技术,检测256例人脑胶质瘤和11例正常脑组织标本。结果 SV40 DNA定位于胶质瘤细胞核,阳性细胞呈弥漫或片灶状分布。胶质瘤SV40 DNA阳性率40.6％（104/256）,正常脑组织均未检测出SV40 DNA,SV40 DNA阳性率与病理分级无关;Tag表达分布与SV40 DNA一致,Tag阳性率28.5％（73/256）较SV40 DNA感染率为低,但Tag表达与病理分级呈显著正相关（P<0.01）。结论SV40感染与人脑胶质瘤病因学密切相关,Tag可能是SV40在胶质瘤发生发展中起作用的重要因素,其机制及生物学意义需进一步研究。

Objective To investigate the location of simian virus 40 (SV40), the expression of large T antigen (Tag) and their possible biological function in carcinogenesis of human brain gliomas. Methods Two hundred and fifty - six cases of human brain glioma and 11 cases of normal brain tissue were studied for SV40 DNA and Tag by using in situ hybridization and immunohistochemisty. Results Hybridization signals were detected within the nuclei of glioma cells and the positive cells distributed diffusively or focally. The SV40 DNA positive rate was 40.6% (104/256) in gliomas while none of the normal brain tissues showed positive results. The correlation between SV40 positive rate and pathological grade was not apparent. The expression and distribution of Tag were consistent with SV40 DNA. Tag positive rate was 28.5% (73/256), which was lower than SV40 DNA positive rate. A significant correlation was found between Tag expression and pathological grade (P < 0. 01). Conclusion A close relation between SV40 infection and etiology of glioma is found. Tag may be a possible etiological agent in the progression of gliomas. The pathogenesis of gliomas and its relationship to SV40 infection remains to be further studied.