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转基因完全人抗体的制备及其抗肿瘤作用研究 被引量：2

Research on the preparation of transgenic fully human antibody and its anti-tumor effects
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摘要 肿瘤导向治疗中,由于所用鼠源性抗体的分子量大、免疫原性强、半衰期长及反复应用易产生人抗鼠抗体(human anti-murine antibody, HAMA)反应等弊端,使其在临床的应用受到很大限制.而采用基因工程技术改造的大部分小分子人源化抗体,由于亲和力较弱、产量低、制备费时和纯化费用高等原因,难以在临床上取得较好的疗效.1994年,美国Abgenix和Genpham两公司报告了利用转基因小鼠制备完全人抗体,从而解决了人体不能被随意免疫这一人抗体制备研究的难题.此后完全人抗体制备技术的不断发展成熟,所获人单克隆抗体(mAb)已具有较强的抗肿瘤活性.
作者 王飞 董军 黄强
机构地区 苏州大学第二附属医院神经外科脑肿瘤研究室
出处 《中华神经外科疾病研究杂志》 CAS 2002年第1期90-91,共2页 Chinese Journal of Neurosurgical Disease Research
关键词 转基因 完全人抗体 制备 抗肿瘤作用
分类号 R979.1 [医药卫生—药品] R392－33 [医药卫生—免疫学]
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参考文献16

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同被引文献48

  • 1张阳德,李浩,李玉坤,龚连生,黄秋,林孙颖,席浩.半乳糖化白蛋白磁性阿霉素纳米粒体外杀伤肝癌细胞的实验研究[J].中华实验外科杂志,2004,21(9):1067-1069. 被引量：10
  • 2连彦军,陈道达,许天文,郑勇斌,黄韬,柯茂林.抗-CEA单抗-β-葡萄糖苷酶偶联物特异性激活苦杏仁苷对LoVo细胞的细胞毒作用研究[J].中国普外基础与临床杂志,2005,12(2):138-141. 被引量：12
  • 3Feodorova V A,Devdariani Z L.Development,characterisation and diagnostic application of monoclonal antibodies against Yersinia pestis fibrinolysin and coagulase[J].J Med Microbiol,2000,49:261-269.
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  • 7Niculescu-Duvaz I.Technology evaluation:gemtuzumab ozogamicin,celltech group[J].Curr Opin Mol Ther,2000(2):691-696.
  • 8Guang Xu,Howard L M.Strategies for enzyme/prodrug cancer therapy[J].Clinical Cancer Research,2001(7):3314-3324.
  • 9Napier M P,Sharma S K.Antibody-directed enzyme prodrug therapy:efficacy and mechanism of action in colorectal carcinomal[J].Clinical Cancer Research,2000(6):765-772.
  • 10Vrouenraets M B,Gerard W M.Targeting of Aluminum (Ⅲ) phthalocyanine tetrasulfonate by use of internalizing monoclonal antibodies:improved efficacy in photodynamic therapy[J].Cancer Research,2001,61(5):1970-1975.

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中华神经外科疾病研究杂志
2002年 第1期

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