三种致病耶尔森氏菌Hsp60基因序列的比较研究

Comparative study on Hsp60 sequences of three pathogenic Yersinae

目的 研究 3种致病耶尔森氏菌Hsp6 0基因序列的差异。方法 根据文献和基因库中的Hsp6 0基因序列设计了两对PCR引物 ,获得Hsp6 0基因的部分序列。将扩增产物纯化回收 ,克隆至 pGEM -T载体后进行测序。 结果 用CLUSTALX软件进行序列分析 ,发现 3种耶尔森氏致病菌的Hsp6 0基因序列较为保守 ,其中鼠疫耶尔森氏菌和假结核耶尔森氏菌的该基因序列在所研究范围内仅相差一个碱基。结论 3种菌的Hsp6 0基因保守 ,不足以在此区间设计种特异性探针。小肠结肠炎耶尔森氏菌与鼠疫耶尔森氏菌和假结核耶尔森氏菌的Hsp6 0基因序列差异较大 。

Aim To investigate the difference between Hsp60 gene of three pathogenic species of Yersiniae Method Two pairs of primers were designed with the aid of DDBJ and literatures,and partial length of Hsp60 sequences were obtained After purified,the amplified products were cloned to T-vector and sequenced Result Hsp60 sequences of three species were so conserved that Y pestis and Y pseudotuberclosis were found to be identical except one base difference,analyzed by CLUSTAL X program Conclusion It is difficult to design species-specific nucleotide probe based on the Hsp60 sequences obtained for differentiating Y pestis and Y pseudotuberculosis,but the difference between Y enterocolitica and Y pestis/Y pseudotuberculosis is great enough to design specific probes for specific identification of Y enterocolitica based on Hsp60 gene