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日本血吸虫成虫培养细胞SDH和LDH细胞化学研究 被引量:14

Cytochemical studies on SDH and LDH of cultured cells from adult Schistosoma japonicum
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摘要 目的 研究日本血吸虫成虫培养细胞琥珀酸脱氢酶 (SDH)和乳酸脱氢酶 (LDH)含量、分布及变化规律 ,了解日本血吸虫成虫培养细胞的能量代谢类型。方法 将虫龄 2 6d的日本血吸虫成虫细胞接种于小盖玻片上 ,培养于RPMI- 16 4 0含2 0 %小牛血清附加常量抗生素的培养基中 ,定时运用Pearson法进行SDH和LDH染色 ,用Olympus-BH2 显微镜观察并拍照 ,用HPIAS - 2 0 0 0图像分析仪测量其含量 ,并作统计分析。结果 日本血吸虫成虫培养细胞均具有SDH和LDH活性 ,两者均分布在细胞质内。培养 1d细胞的SDH和LDH活性最强 ,随着培养时间延长 ,其活性逐渐减弱 ,其中SDH活性下降较快 ,培养 5d大部分细胞SDH活性已极弱 ;而LDH活性下降则较缓 ,培养 5 6d细胞仍具LDH活性。结论 体外培养的日本血吸虫成虫细胞的能量代谢类型与成虫相似 ,既存在三羧酸循环需氧型呼吸链 ,也具有无氧糖酵解 ,但以无氧糖酵解为主。 Aim To recognize the concentration,distribution and variation of succinodehydro genase(SDH)and lactatede hydrogenase(LDH)in the cultured cells from adult Schistosoma japonicum and to make out out the mode of energy meatbolism of the cultured cells.Method The cells from 26 day old adult S.japonicum were inoculated on glass coverslips and cultured in RPMI-1640 containing 20% calf serum and a moderate amount of antibiotics.Thw cells were stained to show the activities of SDH and LDH by using Pearson method regularly,then they were observed and photographed under the microscope(Oympus-BH 2).The concentration of SDH and LDH was measured by using photometer-image analytical instrument(HPIA-2000)and analyzde by statistical method.Results Cultured cells from adult S.japonicum had both of SDH and LDH activities that distributed in cytoplasm.The activities were the strongest in the cells cultrued for l d.Along with prolongation of the culture time,the activities got weaker gradually.The activities of SDH decreased faster than those of LDH.The former was very weak in the cells cultured for only 5 d,while the latter could be measured in the cells cultured for 56 d.Conclusion The mode of energy metabolism of the cultured cells from adult S.japonicum is similar to the adult worm itself.It includs both of aerobic respiration and anaerobic glycolysis,but the blycolysis is the major way in the energy metabolism.
出处 《中国人兽共患病杂志》 CSCD 北大核心 2002年第2期69-71,共3页 Chinese Journal of Zoonoses
基金 武汉市青年科技"晨光计划" 湖北省自然科学基金和教育厅资助项目。
关键词 日本血吸虫 成虫培养细胞 琥珀酸脱氢酶 乳酸脱氢酶 细胞化学 能量代谢 Schistosoma japonicum Cultured cells SDH LDH Cytochemistry
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  • 1董惠芬,蒋明森,杨明义,李瑛,钟沁萍,周述龙.日本血吸虫成虫培养细胞的超微结构观察[J].动物学报,1999,45(1):1-7. 被引量:14
  • 2黄左钺 朱锦洪.日本血吸虫琥珀酸氧化酶的研究[J].Acta Biochinica et Biophysica Sinica(生物化学与生物物理学报:英文版),1962,2:286-286.
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  • 4董惠芬 蒋明森 等.日本血吸虫成虫细胞形态的初步观察.中国动物学会寄生虫专业学会成立10周年纪念论文集[M].,1995.31.
  • 5董惠芬,蒋明森,李瑛,杨明义,周述龙.日本血吸虫细胞培养方法初探[J].水生生物学报,1995,19(4):382-383. 被引量:43

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