淮南工业学院学生肠道菌群调查

Intestinal Flora of Students in Huainan Institute of Technology

目的 探讨正常大学生人群肠道菌群特点 ,为防止和控制因致病菌或条件致病菌引起的肠道腹泻提供依据。方法 用常规细菌培养、SWF A系统生化反应和多价诊断血清分离鉴定肠道菌 ,用沙保氏培养基常规分离鉴定肠道真菌 ,同时选择 1 5种抗生素用Kirby Bauer(K B)法进行体外药物敏感试验。结果 共分离出 7种细菌和 1种真菌 ,即弗劳地枸橼酸杆菌、变形菌、志贺氏菌、哈夫尼亚菌、克雷伯氏杆菌、沙雷氏杆菌、阴沟肠杆菌、白色念珠菌 ,检出率分别为 7.3 %、4 .2 %、7.3 %、2 .5 %、6 .2 %、2 .0 %、3 .9%和 3 .9% ,相互比较差异有显著性 (P <0 .0 1 )。卡那霉素、庆大霉素、痢特灵对多种肠道菌敏感 ,四环素、氨苄青霉素对多种肠道菌耐药。结论 正常大学生人群肠道菌群分布具有多样性 ,以条件致病菌多见 ,当肠道菌群相互比例失调 ,易引起肠道腹泻。部分抗生素对肠道菌产生耐药性。

Objective In order to study the classification of flora in intestine of normal students and to provide the basis for the prevention and control of diarrhea caused by the pathogens and conditioned pathogens in intestine. Methods The bacteria and fungi were isolated from stools and cultivated with the conventional methods, bacteria were identified with the biochemical reaction of SWF-A system and polyvalent diagnostic serum, and fungi were identified by sabourand. The fifteen kinds of antimicrobial susceptibility tests in vitro were made by the method of Kirby-Bauer (K-B). Results Seven strains of bacteria and one strain of fungus were isolated, the positivity rates of Citrobacter freundii, Proteus.Vulgaris, Shigell,Hafnia, Klebsiella. Pneumonia, Enterobacter. Cloacea, Candida albicans were 7.3%, 4.2%- 7.30%, 2.5%, 6.2%, 2.0%, 3.9% and 3.9% respectively, significant differences in the positivity rates were found (P<0.01). Most of intestinal bacteria are sensitive to biograncetina, cidomycin and furazolidone, and the drug resistance of multiple intestinal bacteria to tetracycline and aminobenzylpenicillin occurred. Conclusions The distribution of bacteria in intestine of normal students was varied. Among the bacteria isolated from stools, the conditioned pathogens were more common. As the flora imbalance occurred as diarrhea would take place. The drug resistance of some bacteria had been occurred to some antibiotics.