TLR4胞内区的同源模建

Homology modeling for cytoplasmic region of toil-like receptor 4

目的 为进一步研究新发现的ＴＬＲ４蛋白在ＬＰＳ对机体细胞产生作用的机制，分析其Ｑ结构上的功能区域。方法 同源模建，通过计算机模拟从理论上对ＴＬＲ４胞内区的功能区域进行探讨。结果Ｐｒｏ７１２Ｈｉｓ突变体溶液可及表面的亲疏水性质有明显变化，且相应位置所带的电荷从中性变为正电荷。结论ＴＬＲ４胞内区在发生作用时，起主要作用的是疏水性作用，下一个接头蛋白ＭｙＤ８８与ＴＬＲ４胞内区相互作用的部位应当是一个有较强疏水作用的区域。这样当Ｐｒｏ７１２Ｈｉｓ突变体出现时，由于电荷的排斥，疏水作用无法达到点突变以前的强度，２个蛋白的结合不能得以实现，信号无法继续传递。从而出现对ＬＰＳ的耐受现象。

Objective To get further insight on effect of TLR4 and analyse its structure domain. Methods Homology modeling. Results Great change occurred in solute accessible surface of cytoplasmic region of ThR4. Conclusion Hydrophobicity plays an important role in TLR4 cytoplasmic region. MyD88, the next adaptor protein, should interact TLR4 with a hydrophobic region. In Pm7l2His mutant, they can not form complex because of electronic repulsive force. The signal is blocked, thus LPS tolerance appear.