半乳糖神经酰胺脂质体对HIV-1感染性的影响

Effects of galactosylceramide liposomes on HIV-1 infectivity

目的 研究体外空白及包封抗HIV药物AZT和PFA 的GalCer脂质体 对HIV-1复制的影响。方法 通过HIV p24抗原测定、合胞体抑制实验、 融合阻断实验和对HIV感染细胞的保护作用实验等，观察了空白GalCer脂质体、 AZT-GalCe r脂质体和PFA-GalCer脂质体对HIV-1复制的影响。 结果空白GalCer脂 质体预处理细胞，能促进HIV-1感染CD4+ 细胞；空白GalCer脂质体预处理HIV-1，则抑 制了病毒吸附和结合宿主细胞；GalCer脂质体还具有阻断HIV-1感染细胞与正常CD4+细胞 间的融合作用；GalCer脂质体包封AZT或PFA不能显著提高药物体外抗HIV-1活性。 结论 空白GalCer脂质体预处理细胞，能促进HIV-1感染性；预处理HIV-1，则 抑制了病毒的感染。

Objective To study effects of free and GalCer liposome-encapsulated azidothymidine(AZT) and foscarnet(PFA) on HIV-1 infectivity. Methods Level of HIV-1 p24 antigen, inhibition of syncytium formation, blockage of fusion and protection of HIV-1 infected cells were detected. Results The preincubation of C8166 cells with drug-free liposomes enhanced virus production, and while pretreatment of HIV-1 with drug-free liposomes, the attachment and binding of HIV-1 to cells were markedly inhibited. Liposomes also inhibit membrane fusion between C8166 cells and HIV-1ⅢB chronically infected H9 cells. The liposomal AZT and liposomal PFA gave simila ranti-HIV-1 efficacy compared to free drugs. Conclusion The preincubation of C8166 cells with drug-free liposomes enhanced virus production. While pretreatment of HIV-1 with drug-free liposomes, HIV-1 infectivity was markedly inhibited.