一氧化氮和过氧亚硝基阴离子在家兔脑缺血再灌注损伤中的作用

Effect of nitric oxide and peroxynitrite on ischemia-reperfusion injury of briain

目的 探讨脑缺血再灌注损伤时一氧化氮 (NO)和过氧亚硝基阴离子 (ONOO- )在家兔脑缺血再灌注损伤中的作用。方法 采用夹闭双侧颈总动脉、椎动脉复制家兔脑缺血再灌注模型 ,分别测定对照 (control)组、单纯缺血 (ischemia)组、再灌注损伤 (ischemia -reperfusioninjury ,IR)组脑组织匀浆内超氧化物歧化酶 (SOD)、丙二醛 (MDA)、一氧化氮 (以NO2 - NO3- 代表NO的水平 )、诱导型一氧化氮合酶 (iNOS)水平的变化。结果 缺血再灌注组的NO2 - NO3- 、iNOS、MDA的水平明显高于其他两组 (P <0 .0 5 )而SOD的活性显著降低 (P <0 .0 1)。结论 脑缺血再灌注时脑组织内有大量的NO、ONOO- 产生 ,脂质过氧化增强 ,ONOO-

Objectives: To study the effect of mitric oxide and peroxynitrite on ischemia reperfusion injury of briain.Methods:Copy ischemia-reperfusion injury of briain of rabbit,and observe these change—the content of MDA,NO 2 -/NO 3 -,iNOS and the activities of SOD in the brain tissue.Results: Compared with group ischemia and group control,the contents of Inos,NO 2 -/NO 3 -,MDA increased significantly(P<0.05) and the activites of SOD decreased markedly(P<0.01) in group IR.Conclusions:NO and ONOO - are involved in oxidant-mediated brain injury following ischemia-reperfusion.