神经多肽P物质对中性粒细胞质膜功能蛋白的影响

The Effect of Substance P on Functional Proteins in Human Neutrophil

目的探索神经多肽对中性粒细胞质膜功能蛋白的影响。方法Ｐ物质（ｓｕｂｓｔａｎｃｅ Ｐ，ＳＰ）与中性粒细胞（Ｎｐ）共温育后，用ＮＢＴ和ＨＶＡ方法测定呼吸爆发水平（以Ｏ２·－ 、Ｈ２Ｏ２ 相对量表示），生化法测定质膜酸性磷酸酶（ａｃｉｄｉｃ ｐｈｏｓｐｈａｔａｓｅ，ＡＣＰ）、碱性磷酸酶（ａｌｋａｌｉｎｅ ｐｈｏｓｐｈａｔａｓｅ，ＡＬＰ）活性，荧光标记法测定补体受体３型（ＣＲ３）、白细胞表面共同抗原ＣＤ４５及趋化三肽ＦＭＬＰ受体表达的变化。结果Ｓｐ促进Ｎｐ Ｏ２·－、Ｈ２Ｏ２水平上升，可以使Ｎｐ质膜ＡＣＰ活性降低，而ＡＬＰ无变化，使ＣＤ４５、ＣＲ３和ＦＭＬＰ３种受体的表达量增加。结论神经多肽ＳＰ在促进呼吸爆发的同时，对Ｎｐ质膜功能蛋白的影响具有广泛性和多样性，表明ＳＰ可能影响Ｎｐ参与炎症反应的各个进程。

Objective To explore the effect of substance P(SP)on the functional proteins on plasma mem-brane of neutrophil(Np).Method The response of Np to SP was examined by measuring the level of respiratory burst,the activities of ACP and ALP,the fluoroscopy intensity of CR3,CD45and FM-LP.Results It was found that SP could increase respiratory burst of Np,decrease the activity of acid phosphatase(ACP),but had no effect on alkaline phosphatase(ALP).SP could also promote the amount of CD45,complement receptor type3(CR3)and N-Formyl-Met-Leu-Phe(FMLP)receptors.Conclusion The results showed that the effects of SP on functional proteins in human Np memb -rane were universality and diversity.It implied that SP could affect various inflammation responses in Np.