外源基因在不结球白菜转基因植株后代中的表达

Expression of Foreign Genes in Transgenic Plant Progenies of Nonheading Chinese Cabbage

应用农杆菌介导法将含有豇豆胰蛋白酶抑制剂基因和新霉素磷酸转移酶基因的重组质粒导入不结球白菜地方品种———“中脚黑叶”和“矮脚黑叶”中 ,获得抗虫转基因植株。卡那霉素抗性和抗虫性在转基因植株自交后代T1代植株中发生分离 ;在T2 代中出现 3种不同类型的株系 :抗性纯合、杂合型和敏感性纯合株系。卡那霉素抗性和抗虫性在转基因植株自交后代中的表现基本符合孟德尔显性单基因的分离规律。从T2 、T3 、T4代中选择出抗虫性纯合株系 ,并获得一批对 1～ 2龄鳞翅目小菜蛾、斜纹夜蛾幼虫的校正死亡率达 6 0

Through an agrobacterium mediated transformation,the recombinant plasmids carrying cowpea trypsin inhibitor gene and neomycin phosphotransferase gene were introduced into Brassica campestris ssp chinensis Makino.cv.'Zhongjiaoheiye' and 'Aijiaoheiye'.Insect resistant transgenic plants were obtained in the two varieties.Segregation of kanamycin resistance and insect resistance was observed in T 1 progeny.Three phenotypes:the homozygous line of resistance,heterozygous line of resistance and homozygous line of sensitivity were exhibited in T 2 progeny.The behaviours of kanamycin resistance and insect resistance in progenies of the transgenic plants were conformed to Mendelian Law of the dominant single gene segregation.The homozygous lines of insect resistance were selected from T 2,T 3 and T 4 generation.A number of single plant whose corrected mortalities to the first or second instar larvae of Plutella xylostella (Linnaeus) and Prodenia litura Fabricius reaching 60% were acquired.