NK-κB在EAN中的作用及鼻粘膜耐受的影响

Role of NF-kB in EAN and the effetct of nasal tolerance

目的 :探讨核转录因子NF κB在EAN中的作用以及鼻粘膜耐受的影响。方法 :采用RT PCR和Western blot法检测Wistar大鼠脾脏淋巴细胞IFN γ、TGF β1、IL 10mRNA表达和P6 5蛋白表达。 结果 :IFN γmRNA在发病早期表达明显增加 ,恢复期下降 ;IL 10、TGF β1mRNA在恢复期表达最明显 (P <0 0 5 )。鼻粘膜耐受后大鼠发病率和发病程度减轻 ,IL 10、TGF β1mRNA表达增加 (P <0 0 5 )。P6 5蛋白表达与IL 10、TGF β1mRNA表达呈负相关 ,与IFN γmRNA表达呈正相关。 结论 :P6 5蛋白可能参与EAN的发病 ,鼻服抗原诱导T细胞产生IL 10、TGF β1,通过抑制P6 5蛋白表达这一途径 ,有效预防EAN发生。

Objective:To explore the role of nuclear transcription factor and the effect of nasal tolerance on EAN.Methods:75 male Wistar rats were divided into five groups: Group NS, EAN, EANL, EANM, EANH. The expression of IFN-γ, TGF-β1, IL-10 mRNA and P65 protein were detected in spleen by using of RT-PCR and western blot.Results:The expression of IFN-γ mRNA were the highest at early phase, those of TGF-β1 and IL-10 augmented significantly at recovery phase. In Group EANM and EANH, the severity of clinical signs and the infiltration of inflammatory cells decreased. IFN-γ mRNA and P65 protein expression was lower than that in Group EAN, however, IL-10 and TGF-β1 mRNA increased markedly. At the same time, the expression of P65 protein were positively correlated with those of IFN-γ mRNA, negatively correlated with those of TGF-β1 and IL-10 mRNA.Conclusion:NF-κB may play a vital role in EAN. Nasal administration of antigen may effectively prevent Wistar rat from inducing EAN in some degree, which may associate with the inhibition of NF-κB.