淫羊藿总黄酮与补肾复方对皮质酮大鼠T细胞凋亡相关基因群调控的对比研究

Comparative study of EF and kidney-tonifying recipes on regulatory effect of T lymphocytes apoptosis genes in Corticosterone-treated rats

目的 :对比淫羊藿总黄酮 (EF)与两个含淫羊藿总黄酮的补肾复方及健脾复方对皮质酮鼠模型T细胞凋亡及其相关基因群的调控作用。方法 :采用TUNEL标记的流式细胞技术及荧光实时定量PCR技术对各组大鼠T细胞凋亡及相关基因群mRNA表达水平进行定量与比较。结果 :EF与两个补肾复方都能够降低T细胞凋亡率 ,并对促凋亡及抗凋亡基因群进行适度地调控 ,而健脾方则不能。结论 :EF对皮质酮大鼠T细胞凋亡及其相关基因的调控作用可以代表补肾复方拮抗外源性激素对T细胞的抑制作用。

Objective:To compare the regulatory effect of EF and two Kidney-tonifying recipes as well as Spleen-invigorating recipe on expression of T lymphocytes apoptosis related genes in Corticosterone-treated rats.Methods:To quantify and compare the T lymphocytes apoptosis percentage and the expression the pro-apoptotic and anti-apoptotic genes related to T cell apoptosis in every experimental groups using TUNEL method and flow cytometry assay, as well as the fluorescence real time PCR technology.Results:The percentage of T cell apoptosis and the expression of apoptosis related genes were significantly regulated by EF and two Kidney-tonifying recipes, while the Spleen-invigorating recipe had no significant effect with respect to these parameters.Conclusion:EF can be used to represent the effect of Kidney-tonifying recipes in antagonizing the inhibiting effect of exo-corticosterone with respect to T lymphocytes.