间断缺血对犬骨骼肌存活时限影响的实验研究

Experimental study of intermittent ischemia affects surviving time limit of skeletal muscle in dog

目的 研究间断缺血延长离体骨骼肌存活时限。 方法 以犬股薄肌为模型 ,利用“4C”法、琥珀酸脱氢酶 (SDH)光镜组化、电镜超微结构观察等方法观察离体骨骼肌存活时限。 结果 常温下犬股薄肌的缺血时限为 7h ,低温 ( 4℃ )可以延长骨骼肌的存活时限至 15h ,间断缺血可以延长骨骼肌的存活时限为 2 5h。 结论 间断缺血明显延长骨骼肌存活时限 ,从而可能为断肢保存提供了一种更为理想的方法。

Objective To study proloning the surviving time limit of servered skeletal muscle through intermittent ischemia Methods We use canine gracilis as experimental object to observe its changes by'4C' (colour,capillary bleeding,contractility and consistency of muscle),succinic,dehydrogenase and electron microscope Result (1) At normal temperature the ischemic time limit of skeletal muscle was 7 hours (2) At low temperature (4℃),the surviving time limit of skeletal muscle was 15 hours.(3) However, intermittent ischemia, the surviving time limit of skeletal muscle was 25 hours. Conclusion Intermittent ischemia can prolong the surviving time limit of skeletal muscle obviously So, intermittent ischemia may be used as a new better method of servered limb perservation