植物凝集素荧光标记法检测精子顶体结构的临床意义

The study of human sperm acrosome by means of flurescein-labeled pisum sativum agglutinin technique to evaluate the fertile capacity of human sperm

目的 探寻评估人精子授精能力的简便有效方法。 方法 采用络合异硫氰酸荧光素的植物凝集素 (FITC PSA)进行精子顶体荧光标记 ,分别测定 5 8例不育患者及 4 6例正常生育男子的精子顶体完整率 ,并对其中 14例精液标本 (不育组及正常组各 7例 )进行人卵透明带诱发的精子顶体反应实验 ,对精子顶体完整率与发生顶体反应的百分率的相关关系进行分析。 结果 正常生育组顶体完整率及发生精子顶体反应百分率显著高于不育组 (P <0 .0 1) ,精子顶体完整率与发生精子顶体反应百分率呈正相关 (r =0 .74 68)。 结论 FITC PSA荧光标记法操作简便 ,技术结果可靠 ,特异性强 ,可作为评价人精子授精能力的有效指标之一。

Objective To study human sperm acrosome by means of flurescein labeled pisum sativum agglutinin technique (FITC PSA) to evaluate the fertile capacity of human sperm. Methods The acrosome intact rate was assessed in 58 infertile men and in 46 fertile ones.Human zona pelucida (zp) induced acrosome reaction was detected in 7 infertile and 7 fertile men. Results The acrosome intact rate and the acrosome reaction rate were both higher in the fertile group than those in the infertile group ( P <0.01).There was a high positive correlation between the acrosome intact rate and the acrosome reaction rate (r=0.7468). Conclusions FITC PSA flurescent staining is a simple, reliable and valuable means to evaluate the fertile capacity of human sperm.