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重组人睾丸前列腺素D合成酶DNA免疫鸡的初步研究 被引量：2

Preliminary Study on Hens Immunized with Recombinant Human Testis Prostaglandin D Synthase DNA
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摘要 目的 :证实用重组人睾丸前列腺素D合成酶 (rhtL PGDS)DNA免疫鸡可获得相应的抗体。　方法 :用重组质粒 pGEX 2T/htL PGDSDNA(10 0 μg)免疫鸡 ,隔 2周加强 1次 ,共 2次。抽取鸡血分离血清 ,以原核表达的rhtL PGDS作为抗原 ,琼脂糖双向免疫扩散法和ELISA法检测鸡血清中有无抗L PGDS抗体及其效价。　结果 :琼脂糖双向免疫扩散法证实鸡血清中存在抗L PGDS抗体 ,ELISA法检出其效价为 1∶2 0 4 8。　结论 :用重组质粒pGEX 2T/htL PGDSDNA免疫鸡制备抗L Objectives: In order to identify whether the hens immunized with recombinant human testis prostaglandin D synthase (rhtL PGDS) DNA can produce anti L PGDS antibody. Methods: The serum were got from the hens immunized with recombinant plasmid pGEX 2T/htL PGDS DNA (100μg) every 2 weeks for 2 times. The exist of anti L PGDS antibody and its titer were tested with agarose dual immunodiffusion and ELISA with rhtL PGDS as antigen. Results: The serum anti L PGDS antibody in hen immunized with pGEX 2T/htL PGDS DNA were confirmed and its titer tested by ELISA was 1∶2 048. Conclusions: It is feasible to produce anti L PGDS antibody by immunizing hens with recombinant pGEX 2T/htL PGES DNA.
作者 陆金春 黄宇烽 张锡然
机构地区 武警南京医院检验科 南京军区南京总医院生殖遗传室 南京师范大学生命科学学院
出处 《中华男科学杂志》 CAS CSCD 2002年第1期22-24,共3页 National Journal of Andrology
基金 武警总部基金资助 (编号WKH2 0 0 10 3 6)
关键词 前列腺素D合成酶 DNA免疫 睾丸 琼脂糖双向免疫扩散法 ELISA法 Prostaglandin D synthase DNA immunization Testis Human
分类号 Q783 [生物学—分子生物学]
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参考文献5

二级参考文献30

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共引文献19

同被引文献6

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  • 6汪俊军,庄一义,刘小传,李勇,张宏娟.抗胆固醇酯转运蛋白单克隆抗体的制备及其应用[J].临床检验杂志,2001,19(4):200-202. 被引量：4

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二级引证文献15

中华男科学杂志
2002年 第1期

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