外源性bFGF对大鼠创伤性脑损伤后学习记忆功能障碍的治疗作用

Effects of exogenous bFGF on cognitive dysfunction following traumatic brain injury in rats

目的 探讨外源性碱性成纤维生长因子 (bFGF)对脑创伤后学习记忆功能障碍的治疗作用。方法 以Marmarou’s方法制作大鼠重型弥漫性脑创伤模型 ;利用HE染色、原位细胞凋亡检测及Morris水迷宫技术 ,对大鼠伤后海马区神经细胞的病理改变及空间学习记忆功能的变化进行动态观察。结果 伤后给予外源性bFGF可明显抑制大鼠海马CA2 3区神经细胞的坏死、凋亡过程 ;治疗组Morris水迷宫测试潜伏期于伤后第 8天及第 1 0天较创伤组明显缩短。结论 海马是学习记忆形成的重要脑功能结构。外源性bFGF通过抑制伤后海马神经细胞缺失 。

Objective To investigate the protective effect of exogenous basic fibroblast growth factor(bFGF)on cognitive dysfunction following traumatic brain injury in rats.?Methods The model of severe diffuse traumatic brain injury was established in rats by using of the method found by Marmarou.After traumatic brain injury,the histopathological damage in the hippocampus of rats was observed by means of H&E and TUNEL stain respectively, and spatial cognitive dysfunction was evaluated by using the Morris water maze (MWM).?Results The treatment with exogenous bFGF could prevent neuronal necrosis of hippocampal CA2 3 and apoptosis obviously ,and attenuate learning and memory dysfunction in the Morris Water Maze significantly postinjury compared to vehicle treatment.?Conclusion Hippocampus is an important functional structure of learning and memory. It is possible that the attenuation of cognitive dysfunction resulting from bFGF in the present study was associated with the function of increased neuronal survival in the hippocampus.