期刊文献+

脐血HLA-B15组血清学与DNA定型方法的比较研究 被引量:3

下载PDF
导出
摘要 目的 探讨血清学方法对脐血HLA B1 5特异性组分型存在的误差 ,及应用PCR SSP方法分型的可行性。方法 分别采用微量淋巴细胞毒实验和PCR SSP方法对 1 37份脐血做分型对比研究。结果 DNA分型成功率 1 0 0 % ,特异性、敏感性好 ,血清学对B1 5组分型总误差 1 5 % ,主要发生在B62、B75、B1 51 1 / 1 50 8/ 4
出处 《中国输血杂志》 CAS CSCD 2002年第1期23-26,共4页 Chinese Journal of Blood Transfusion
  • 相关文献

参考文献11

  • 1Maduigal JA,Arguello R,Scott I,etal.Molecular histocompatibility typing in unrelated donor bone marrowtransplantation.Blood Rev,1997,11:105
  • 2Hurley CK,Wade JA,Oudshoom M,et al.Tissue Antigens,1999,53:394
  • 3Lee JH,Lias M,Deng CT,et al.A one step monoclonal antibody typing procedure thatsimplifies HLA class I and class Ⅱ typing.Tissue Antigens,1994,44:34
  • 4Sadler AM,Petronzelli F,Krausa P,et al.Low-resolution DNA typing for HLA-B usingsequence-specific primers in allele or group specific ARMS/PCR.Tissue Antigens,1994,44:148
  • 5Bunce M,Oneill CM,Barnardo MCNM,et al.Phototyping: comprehensive DNA typing forHLA-A,B,C,DRB1,DRB3,DRB4,DQB1 by PCR with 144 prime mixes utilizing sequence-specificprimers.Tissue Antigens,1995,46:335
  • 6Lin L,Tokunga K,Tanaka H,et al.Further molecular diversity in the HLA-B15group.Tissue Antigens,1996,47:265
  • 7Hildebrand WH,Domena JD,Shen SY,et al.HLA-B15:A widespred and diverse family ofHLA-B alleles.Tissue Antigens,1994,43:209
  • 8Kostyu DD,Hannick LI,Traweek JL.HLA class I polymorphism:structure and fuction andstill questions.Hum Immunol,1997,57:1
  • 9Magira E,Beznik-Cizman B,Monos D.HLA-B*1559:a hybrid allele including exon 2 ofHLA-B35 and exon 3 of HLA-B15 and serologically typed as B35.Tissue Antigens,2000,56:460
  • 10Barnardo MC,Welsh KI,Vilches C,et al.Allele-specific HLA-B15 typing by PCR-SSP andits application to four distinct ethnic populations.Tissue Antigens,1998,51:293

同被引文献5

引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部