摘要
以低温冰箱和液氮作为冷源,对四种淡水养殖鱼类的胚胎进行低温保存试验。采用10%二甲亚砜(DMSO)为抗冻剂,鲤鱼胚胎冷冻至-5℃、-10℃和-20℃,复温后的最高成活率分别为96%、90%和31%;团头鲂胚胎冷冻至-10℃、-15℃和-20℃,复温后的最高成活率分别为43%、20%和12.5%;青鱼胚胎冷冻至-5℃、-10℃和-11℃,复温后的最高成活率分别为100%、40%和0%;白鲢胚眙冷冻至-7℃,复温后的最高成活率为19.6%。造成胚胎损伤、致死的主要原因是胚胎在降温过程中细胞严重失水。
Embryos of common carp and blunt snout bream were lowered the temperature to —20℃, the survival rates were 31% and 12.5% respectively. Embryo of black carp at —11℃, the survival rate was zero. 10% dimethyl sulfoxide (DMSO) was as a protective agent, Cell dehydration during slow freezing was the main reason for embryo damage and mortality.
出处
《水产科技情报》
1991年第3期68-71,共4页
Fisheries Science & Technology Information
关键词
鱼类
胚胎
低温保存
淡水养殖
Freshwater cultured fishes Embryo Cryopreservation Protective agent
