氧化型低密度脂蛋白诱导猪主动脉平滑肌细胞凋亡

Oxidized Low Density Lipoprotein Induces Apoptosis of Porcine Aortal Smooth Musc le Cells

为了探讨氧化型低密度脂蛋白是否引起血管平滑肌细胞凋亡 ,把体外培养的猪主动脉平滑肌细胞与 15mg/L的氧化型低密度脂蛋白孵育 72h ,分别使用荧光染色技术和激光共聚焦显微技术观察细胞核的变化 ,应用流式细胞仪鉴别凋亡细胞。结果发现 ,15mg/L的氧化型低密度脂蛋白作用 72h后 ,荧光显微镜下可见染色质高度凝集 ,有核碎裂现象 ;激光共聚焦显微镜下见染色质固缩 ,核变小 ;流式细胞术鉴别为凋亡细胞。此结果提示 ,氧化型低密度脂蛋白能诱导血管平滑肌细胞发生凋亡 ,有典型的形态学改变。

Aim To investigate whether oxidized low density l ipoprotein induces apoptosis of vascular smooth muscle cells. Methods The cultured porcine aortal smooth muscle cells incubated with oxidized low density lipoprotein of 15 mg/L for 72 hours in vitro. Then the samples were analyzed by fluorescence microscope?confocal microscope system and flow cytometry. Results Apoptosis was triggered by incubated with oxidized low density lipoprotein accompanying with apparently morphological changes. Conclusion In the process of which oxid ized low density lipoprotein induced vascular smooth muscle cells apoptosis, the abnorm al metabolism of cellular cholesterol may be have relation to apoptosis.