MNNG对日本血吸虫成虫细胞鸟氨酸脱羧酶活性作用的初步研究

Effect of MNNG on Ornithine Decarboxylase Activity in Cells from Adult Schistosoma japonicum

目的 观察 N-甲基 - N-硝基 - N-亚硝基胍 (MNNG)诱导后日本血吸虫成虫细胞鸟氨酸脱羧酶 (ODC)活性的变化。 方法 将日本血吸虫成虫剪碎后 ,收集成虫细胞 ,培养 1wk后依次用 3μg/ ml的 MNNG处理 4 8h,用 RP-MI- 16 4 0加 10 %小牛血清及常量抗生素的培养基清洗数次并继续培养。对照组不用 MNNG处理。分光光度法测定ODC活性。 结果 MNNG诱导后第 2、3周 ODC活性显著增高。 结论 日本血吸虫成虫细胞内存在 ODC活性 ,MNNG具有增强

Objective To observe the change of ornithine decarboxylase(ODC) activity in cells from adult Schistosoma japonicum after the cells were treated with N\|Methyl\|N\|Nitro\|N\|Nitrosoguanidine (MNNG). Methods The cells were treated with MNNG at a concentration of 3 μg/ml for 48 hours after the cells being incubated for one week. The cells were then cultured with RPMI\|1640 containing 10% calf serum. ODC activity was detected with spectrophotography. Results ODC activity rose significantly in two to three weeks after the cells were treated with MNNG. Conclusion There was ODC activity in cells from adult S.japonicum and MNNG has an effect to reinforce ODC activity in the cells.