高效液相色谱法测定不同产地黄芩中黄酮化合物的含量

Detrmination of Flavone for Scutellaria baicalensis from Different Areas by HPLC

目的 :测定不同产地黄芩中黄酮类化合物的含量 ,以评价其道地性。方法 :高效液相色谱法 (HPLC)。结果 :17个产地 2 5份样品中黄芩苷含量变化在 6 %～ 19%、汉黄芩苷在 2 %～ 8%、黄芩素在 0 .1%～ 1.6 %、黄芩新素在 0 .0 1%～ 0 .2 %、汉黄芩素在 0 .0 1%～ 0 .3% ,粘毛黄芩素、木蝴蝶素为痕迹量或未检出。结论 :单纯以黄芩苷的含量来评价其质量是不够的 ,建议将黄芩苷与汉黄芩苷的含量比值在 3以下、黄芩苷与黄芩素、黄芩苷与汉黄芩素的含量比值在 2 0～ 5 0等参数列为质量控制指标。

Objective :To describe the difference between native and nonative herbs by determining contents of seven kinds of flavone for twenty -five samples from seventeen areas.Method: HPLC. Fluid phase: MEOH-H 2O-CH 3COOH(ICE) (41∶59∶0.2)and (50∶50∶0.2).Detection wavelength:275. Result:The contents of baicalin are 6%～9%,wogenin are 2%～8%,baicalein are 0.1%～1.6%,neobaicalein are 0.01%～0.2%,wogonin are 0.01%～0.3%,visidulin and oroxylin are trace amounts or undetected.Conclusion: The native and nonative herbs have no distinct differerce in absolute component ratio.The ratio of baicalin and wogenin is under three .The ratio of baicalin and baicalein ,baicalin and wogonin is between twenty and fifty.