温病急性热瘀证与血小板GMP-140、D-二聚体的相关性实验研究

The Experimental research on the relation between acute heat statis syndrome in seasonal febrile disease and GMP - 140,D - D

目的:观察湿病病变过程中血瘀的轻重程度。方法:本文将20只新西兰白兔随机分为对照组和模型组各10只,将大肠杆菌内毒素剂量由小及大,每间隔5小时从模型组家兔耳缘静脉注射一次(攻毒),剂量依次为1μg/kg、15μg/kg、30μg/kg、250μg/kg。对照组同时从耳缘静脉注射生理盐水。每次“攻毒”后1.5小时采血,检测血浆D-二聚体(D-D),血小板GMP-140。结果:第一次“攻毒”后血小板GMP-140分子数明显增高,(与对照组比P<0.01)。第二次“攻毒”后D-D浓度开始升高(与对照组比P<0.01),其后上述指标随时间推移和“攻毒”剂量加大而递增。提示:温病病变过程中GMP-140能反映温病早期的“血瘀”变化,而D-D在温病后期变化最明显。两项指标能客观的反映温病病变过程中“血瘀”轻重程度。

Objective:To investigate the degree of blood stasis in seasonal febrile disease. Method:20 rabbits are divided randomly into experimental group and control group, 10 per group.Each rabbit in experimental group was injected with endo-toxin from its aural marginal vena( named: evil - attacking) every 5 hours by the dose of 1μg/kg,15μg/kg,30μg/kg and 250μg/kg gradually from small to large dose. And each rabbit in control group was injected with NS. Blood samples were taken from all rabbits' hearts 1.5 hours after each evil - attacking to examine the levels of D - dimer(D - D)and a -granule membrane protein( GMP - 140) . Results: the level of GMP - 140 increased obviously compared to the control group alter the first evil - attacking, level of D - D statistically significantly increased ( p < 0.01) , and the two markers changed proportionally to the time and the dose of evil- attacking. Conclusion: GMP - 140 can show the early change of blood stasis, and D-D changed significantly at the later stage of seasonal febrile disease. GMP - 140 and D-D can regarded as two important markers suggesting the degree of blood stasis in seasonal febrile disease.