辛伐他汀诱导大鼠血管平滑肌细胞凋亡及对凋亡相关基因表达的影响

Simvastatin induced apoptosis and its effect on apoptosis-related gene expression in rat vascular smooth muscle cell

目的 :观察辛伐他汀诱导大鼠血管平滑肌细胞 (VSMC)凋亡及可能参与其中的凋亡相关基因。方法 :贴块法体外培养 VSMC,以透射电子显微镜技术、流式细仪 PI/Annexin 双重染色等鉴定细胞凋亡 ;Western印迹杂交法检测 Bax、Bcl- 2蛋白表达及 caspase- 3的激活情况。结果 :加入 30μmol/L辛伐他汀 2 4 h后电镜显示细胞呈典型的凋亡形态 ,PI/Annexin 染色流式细胞仪检测凋亡细胞占 (35 .5± 5 .8) %显著多于对照组 (15 .1± 5 .0 ) %(P<0 .0 5 ) ;辛伐他汀不改变 Bcl- 2蛋白的表达 ,而使 Bax蛋白表达增高并激活 caspase- 3。结论 :辛伐他汀能诱导VSMC产生凋亡 ,并与 Bax表达增高及 caspase-

Objective: To observe the influence of simvastatin on the apoptosis of vascular smooth muscle cells(VSMC) and its effects on the expression of apoptosis related genes. Methods: The presence of apoptosis was detected by electron microscope and flow cytometry assessment of PI/Annexin Ⅴ stain; The protein levels of Bax, Bel 2 and activation of caspase 3 were examined using Western blot technique. Results: After treatment with 30 μmol/L simvastatin for 24 h, apoptosis were identified with electron microscope in VSMC and flow cytometry showed that rate of apoptosis in simvastatin group (35.5±5.8)% was singificantly higher than that in control group (15.1±5.0)%. Western blot analyses revealed that the apoptosis process was associated with upregulation of Bax protein and activation of caspase 3, but not with Bel 2 expression. Conclusion: Simvastatin can induce apoptosis in VSMC in associated with induction of bax and activation of caspase 3.