异育银鲫溶血性腹水病病原的研究

ON THE PATHOGENIC BACTERIA OF THE HEMOLYTIC ASCITESOSIS OF ALLOGYNOGENETIC CRUCIAN CARP

本文对上海市郊县和江苏省吴江县的异育银鲫溶血性腹水病,进行了病原分离和人工感染试验。对致病菌株进行了细菌学鉴定,其分别为运动型气单胞菌的苏伯利气单胞菌(Aeromonas sobria)和嗜水气单胞菌(A.hydrophila)。通过对人工感染病鱼血清内毒素(En-dotoxin)的测定,并用菌体破碎液注射健康鱼体试验,初步认为病原产生和释放的内毒素类物质,是使鱼类致病和死亡的主要原因之一。

Hemolytic ascitesosis of allogynogenetic crucian carp occured rece-ntly both in the suburb of Shanghai and Wujian County (Jiashu Province). Experi-ments of isolation and artificial infection tests have been done and the virulent pat-hogenic bacteria strains such as N-1-2,89-7-14, D-Ⅱ-1 were available. Accordingto the morphological features of the bacterium, cultural characteristics,physiologicaland biochemical reacts, and the G+C Mol % of these strains, it was identified to beAeromonas sobia and A. hydrophila of moving Aeromonas. By applying the methods of tachypleus amebocyte lysate, serum of five ill fishwhich were artifecally infected by N-1-2 strain has been given the qualitative ana-lysis of endotoxin, and the result is positive. The same experiment has been appliedto the serum of four other healthy fish, and the result is negative. Then the disrup-ted bacteria cell suspension of the strain is injected to healthy fish, the result ob-tained are the same as those caused by pathogeny cells infection, so it is consideredthat the pathogony during its growing, reproduction and self-dissolving within thefish produces and liberates such a substance as endotoxin which caused the fish di-sease.