摘要
目的研究苦参碱(matrine,Mat)对人增生性瘢痕成纤维细胞细胞周期和细胞核内增殖性抗原(proliferatingcellnuclearantigen,PCNA)的表达影响。方法将不同浓度梯度Mat作用于体外培养的增生性瘢痕成纤维细胞,利用通过流式细胞仪检测其细胞周期、DNA相对含量,利用用免疫组织化学的检测PCNA的表达。结果不同浓度梯度的Mat作用增生性瘢痕成纤维细胞72h后,出现G0～G1期细胞数量明显增加,S期细胞、G2～M期细胞明显减少,DNA相对含量无明显变化,PCNA表达逐渐减弱,(P<0.01)。结论Mat能明显抑制增生性瘢痕成纤维细胞的增殖活性和细胞的有丝分裂,但对DNA含量无明显影响。
Objective To study the effect of Matrine on the cell cycle , DNA content and the expression of PCNA from scar derived fibroblast. Methods The fibroblasts derived from 3～5 generations of the cultured hypertrophic scar tissues. Compared the cells that treated with different concentration gradient matrines and the control groups which treated with normal saline.by the cell cycle, DNA content and the expression of PCNA performed flow cytometry and examed by immunohistochemical method respectively.Results Compared with the control groups the distribution in cell cycle was showed that the percentage of G2～M phase and S phase decreased, the percentage of G0～G1 phase cells increasd significantly (P< 0.01).The contents of DNA were stabilized,the expression of PCNA was under the controls.Conclusion Matrine could significantly block the mitosis and proliferation of fibroblasts derived hypertrphic scar unchanged the contents of DNA.
出处
《中国临床康复》
CSCD
2002年第6期806-807,共2页
Chinese Journal of Clinical Rehabilitation
