反相高效液相色谱-荧光法测定血清中异丙酚浓度

Determination of Propofol Concentration in Human Serum With RP-HPLC

目的 :建立反相高效液相色谱 -荧光检测法测定血清中异丙酚浓度的方法。方法 :使用ODSC18 柱 ,以甲醇 -水 (75∶25)为流动相 ,激发波长270nm ,发射波长295nm。结果 :本法测定的线性范围为0 0375～8μg/ml ,r=0 9996 ,日内和日间RSD<5 % ,平均回收率为83 98 %。结论 :本法简单、快速 。

OBJECTIVE:To develop a RP-HPLC method for determining serum concentration of propofol METHODS:Using ODS C18 column as fixed phase,a mixture of methanol and water(75∶25) as mobile phase,excitation wavelength 270nm,emission wavelength 295nm RESULTS:The linear range was 0 0 375～8 0μg/ml,r=0 9 996 The within-day and between-day RSDs were less than 5%,the average recovery was 83 98% CONCLUSION:This is a good method to monitor propofol serum concentration