心肌成纤维细胞NOS-NO系统活性的变化及其与AVP的关系

Changes of NOS-NO system activity in neonatal rat cardiac fibroblasts with and without arginine vasopressin

目的 :探讨无干预和血管升压素 (AVP)干预条件下 ,心肌成纤维细胞 (CFs)的一氧化氮合酶—氧化氮 (NOS-NO)系统活性的变化。方法 :胰酶消化法分离、培养新生 SD大鼠 CFs,采用硝酸还原酶法和分光光度法观察无干预和 AVP干预条件下 ,不同培养时间对 CFs的 NOS- NO系统活性的影响。结果 :1无干预条件下 ,CFs的 NO含量和 NOS活性随培养时间延长而增高 ,其中 36 h(4 2± 5 μmol· L- 1 ,37± 5 U· m L- 1 )显著高于 6 h(14± 3μmol·L- 1 ,10± 4U· m L- 1 )以及 12 h(2 1± 3μm ol· L- 1 ,15± 3U· m L- 1 ) (均 P<0 .0 5 )。 2 AVP干预条件下 ,CFs的NO含量和 NOS活性也随培养时间延长而增高 ,其中 2 4h(6 5± 6 μmol· L- 1 ,70± 4U· m L- 1 ) ,36 h(6 2± 1μm ol· L- 1 ,6 9± 6 U· m L- 1 )都显著高于 6 h (34± 4μmol· L- 1 ,36 +2 U· m L- 1 )以及 12 h的 (4 5± 4μmol· L- 1 ,45± 1U· m L- 1 ) (均 P<0 .0 5 )。 3AVP干预条件下 CFs的 NO含量和 NOS活性均较无干预条件下显著增高 ,且 NO含量随 NOS活性增高而增高 ,二者呈显著正相关 (无干预条件下 r=0 .837,P<0 .0 1;AVP干预条件下 r=0 .936 ,P<0 .0 1)。结论 :AVP提高 CFs的 NOS- NO系统活性 ,CFs的 NOS- NO系统活性与培养和 AVP?

AIM:To investigate the changes of nitric oxide synthase-nitric oxide(NOS-NO) system activity in neonatal rat cardiac fibroblasts(CFs) with and without arginine vasopressin(AVP). METHODS: CFs were isolated by trypsin digestion method. Nitric acid reductase method and spectrophotometry were used to detect the NO contents and NOS activity respectively at different time points with or without AVP. RESULTS: ①NO contents and NOS activity increased in a time-dependent manner without AVP(control group). NO contents and NOS activity of 36 h(42±5μmolL-1aa,375UmL-1aa) were higher than those of 6h(143|ìmolL-1aa, 104UmL-1) and those of 12h(213|ìmolL-1aa,153UmL-1)(both *'P*'<0.05). ②NO contents and NOS activity increased in a time-dependent manner with AVP(AVP group). NO contents and NOS activity of 24h(656 |ìmolL-1,704 UmL-1) and of 36 h(622 |ìmolL-1aa, 696 UmL-1) were both higher than those of 6 h(344 |ìmolL-1aa,362 UmL-1aa) and those of 12 h(454 |ìmolL-1aa, 451 UmL-1)(both *'P*'<0.05). ③NO contents and NOS activity of AVP group were both higher than those of control group. NO contents increased with the enhancement of NOS activity, and there was significant positive relevance between them(control group: *'r*'=0.837, *'P*'<0.01; AVP group: *'r*'=0.936, *'P*'<0.01). CONCLUSION:AVP increases NOS-NO system activity in neonatal rat CFs. Whether with or without AVP, the NOS-NO system activity increases in a time-dependent manner.