摘要
本实验利用绿色荧光蛋白 ( GFP)与嗜上皮启动子 EDL 2连接 ,运用显微注射技术将EDL 2 -GFP注射入爪蟾受精卵以制备转基因蛙 ,根据 GFP在蛙体内的表达情况 。
In present study, green fluorescence protein(GFP) gene was linked to epithelium specific promoter EDL2 to construct reporter plasmid, then EDL2 GFP was microinjected into zygotes of Xenopus. According to the expression status of GFP in vivo, the tissue specific character of the promoter can be decided preliminarily.
出处
《动物医学进展》
CSCD
2002年第2期57-58,共2页
Progress In Veterinary Medicine