摘要
将新城疫病毒 (NDV)F4 8E9株融合蛋白 (F)基因 170 0bp克隆到真核表达载体 pcDNA3 中 ,构建成表达F基因的载体 pc3F。然后将包含F基因及其上游的细胞巨化病毒启动子和增强子的 3 80 0bpDNA片段再克隆入包含火鸡疱疹病毒 (HVT)非必须片段载体 pTK2 B中 ,构建成功含有F基因表达盒的HVT转移质粒载体 pTK3 F。经限制性内切酶酶切分析 ,F基因表达盒的插入方向与pTK2 B中TK基因转录方向一致。
The NDV F48E9 strain Fusion Protein gene was cloned into the multi clone site of eukaryotic expression vector pcDNA 3 to form pc3F. 3800 bp DNA fragment of F gene with CMV promotor and enhancer upstream was cloned into the single NheI site of HVT nonessential fragment vector, and the HVT transferring vector containing F gene cassette was constructed. The restriction analysis indicated that the direction of TK gene cassette insertion is same with that of TK gene transcription. The result is the basis of obtaining recombinant HVT expressing NDV F gene.
出处
《中国兽医科技》
CSCD
北大核心
2001年第8期5-8,共4页
Chinese Journal of Veterinary Science and Technology
