摘要
用国际 4个稻瘟病菌的标准菌株 ,测定我国 17省、市 378个稻瘟病菌的交配型及其能育菌株的地理分布 ,并用 13对引物的PCR产物对其 73个能育菌株进行SCARs分析 ,初步明确了我国稻瘟病菌交配型的地理分布及其能育菌株的亲缘关系 ,并对该菌有性生殖在田间存在的可能性进行了讨论。
Three hundred and seventy eight isolates of Magnaporthe grisea were collected from 17 provinces (cities) in China and their geographic distribution of mating type and its fertility was tested with 4 standard isolates, KA3 and TH12 (Mat1.1) and Guy11 and TH16 (Mat1.2) provided by CIRAD. Seventy three fertile isolates of them were tested with polymorphic SCARs analysis by use PCR markers of 13 primer pairs. Preliminary results showed that the geographic distribution of M. grisea existed among isolates from the same location as well as different location and its relative relationship between fertile isolates of the fungus in China. The existence of sexual reproduction of M. grisea was explored in the field as well.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第3期256-263,共8页
Scientia Agricultura Sinica
基金
国家科技部生物技术
开发中心"中法先进研究计划"生物领域合作项目 (国科生字 [1997] 15 5号 )
关键词
稻瘟病菌
能育菌株
地理分布
SCARS
亲缘关系
交配型
Magnaporthe grisea
Fertile isolates
Geographic distribution
SCARs
Relative relationship
