短芒大麦耐盐碱新品系的生理生化和分子生物学分析

Physiological-biochemistrical and Molecular Biological Analysis of Salt-tolerant New Lines of Hordeum brevisubulatium(Trin.)Link

以经60 Co物理诱变种子、EMS诱变和耐盐反复筛选后已稳定 6代的短芒大麦耐盐碱突变体N1、T3 、T4、T5及其亲本野生短芒大麦为材料 ,进行耐盐碱生理生化指标 (脯氨酸、质膜透性 )测定及RAPD分析。发现突变体与野生型相比 ,不仅在生理生化指标上有明显的差异 ,而且在DNA水平上发生了突变。研究结果为耐盐碱突变体的真实性提供了有力的证据 ,表明突变体N1、T3 、T4、T5是耐盐碱短芒大麦新品系。

Four Hordeum brevisubulatium(Trin.)Link salt tolerant mutants (N 1,T 3,T 4,T 5) derived from 60 Co irradiation, EMS induction and salt tolerance selection and their original line (CK), were used as materials and all the mutants have inherited stably for 6 generations. Distinct differences were revealed between the mutants and original line by using physiological biochemistrical indexes (proline, electric conductivity) and molecular marker (RAPD). The result showed that compared with the original line, the mutants varied not only on the salt tolerant indexes level, but also on the DNA level, which provided a strong evidence for the truth of the mutants. This study showed that these mutants (N 1,T 3,T 4,T 5) were salt tolerant new lines of Hordeum brevisubulatium(Trin.)Link.