脱乙酰化几丁质的乙酰化程度对诱导油菜抗性的影响

Effest of Partially N-acetylated Chitosans on Induction of Resistance in Brassica napus L.

对甘蓝型油菜“西农长角”发芽 5d的幼苗子叶进行涂抹抗性诱导效应及基机理的影响研究表明 ,脱乙酰化几丁质对抗性的诱导与水扬酸 (SA)相似 ,但乙酰化程度对诱导效果有显著的影响并体现出一定的规律性。苯丙氨酸解氨酶 (PAL)活性随乙酰化程度增加而提高 ;而几丁质酶活性则随乙酰化程度增加而下降 ,只有完全脱乙酰化 (D .A .为 0 % )的 10B和GC能诱导酶活性提高 ,而D .A .为 10 %、2 0 %、30 %的 7B、8B和 9B则表现出抑制作用 ;过氧化物酶(POD)活性都能被诱导而比对照高 ,但乙酰化程度的影响不显著。

The effects of four partially N acetylated chitosan and Glycol chitosan on induction of resistance in Xinong Changjao, a variety of Brassica were studied. Results showed that chitosan was similar to salicylic acid (SA) in induction of resistance, but the induction was influenced by the degree of acetylation of chitosan. Fully deacetylated chitosan, 10B and GC, induced high chitinase activity, but partially acetylated chitosan, 7B, 8B and 9B, inhibited chitinase activity. Phenyalanine ammonialyase (PAL) was also induced and its activity increased with the increasing of acetylation, 7B induced highest PAL activity among all chitosans. All chitosans induced peroxidase (POD) in similar level.