摘要
通过悬浮培养和 0 5 μmol LRA诱导 ,建立了定向诱导ES细胞向神经元样细胞分化的体系。在该体系中 ,神经元样细胞于类胚贴壁后 4 8h之内出现 ,4~ 6d时达到最多 ,8~ 10d后减少。由ES细胞分化而来的神经元样细胞具有典型的形态特征 ,并且可以与NF抗体发生特异反应。
Via suspension culture and 0.5 μmol/L RA induction,we established an in vitro system to differentiate ES cells directly into neuron\|like cells.In this system,neuron\|like cells first appeared within 48?h after the embryoid bodies were plated,and were most abundant 4\|6 days after plating,while decreased 8\|10 days later.Neuron\|like cells differentiated from ES cells had typical morphologic properties,and specifically reacted with NF antibodies.
出处
《北京大学学报(自然科学版)》
CAS
CSCD
北大核心
2002年第2期192-196,共5页
Acta Scientiarum Naturalium Universitatis Pekinensis
基金
国家自然科学基金重点资助项目 ( 3 973 0 2 5 0 )