酵母双杂交系统检测金属硫蛋白(MT)间的相互作用

Detection of Interactions among Metallothioneins by Yeast Two-Hybrid System

金属硫蛋白 3(MT 3) ,又称神经生长抑制因子 ,为金属硫蛋白家族中惟一具有生物活性的成员。为了验证其在细胞内是否形成二聚或多聚体 ,构建酵母双杂交系统用于检测。结果表明 :MT 3与MT 3之间在酵母细胞内能发生相互作用 ,进一步用酵母双杂交实验还表明MT 3与MT 1间也存在弱相互作用。由此提示 ,在机体细胞中 ,MT 3可以以同源或异源二聚体的形式存在。

Metallothionein\|3(MT\|3),previously named as growth inhibitory factor,is the unique member of the metallothionein family that demonstrates bioactivity in vitro .To test if MT\|3 dimerizes or polymerizes itself in vivo ,the yeast two\|hybrid system was established.In yeast two\|hybrid system,MT\|3 interacts with MT\|3.A further two\|hybrid test also indicated that MT\|3 weakly interacted with its isoform MT\|1.These primary results suggest that MT\|3 probably trend to homodimerize or heterodimerize in vivo .