草履虫类核纤层蛋白的初步研究

A Preliminary Investigation on the Lamin-like Proteins in Paramecium

本文以DGD包埋去包埋技术对草履虫的核纤层通过透射电镜和免疫荧光、分子杂交等技术进行了观察。结果显示 ,在核周存在由 10nm纤维组成的核纤层 ;免疫荧光结果表明 ,在核周呈阳性反应 ,并在其表皮、口器等部位呈交叉的阳性反应 ;蛋白分子杂交的反应带在

Paramecium caudatum was first processed by DGD embedment and embedment\|free technique and general technique for EM morphology.A perinuclear structure consisted of interacted filaments (we called lamina\|like structure) was observed.Then using Western Blot assay,we found a lamin\|like component band of 68?kD protein in the three\|step\|fractionated cells.To investigate the distribution of the lamin\|like protein in cells,a immunofluorescence experiment for in situ hybridization was designed using goat anti\|lamin protein antibodies as the probes.The results revealed that the positive reactivity presented at different part of the cells.The perinuclear cross\|actions were distinct,and cross\|action with the oral apparatus and the cortex were also obtained.